Nutrient and Hormonal Regulation of the Threshold of Glucose-Stimulated Insulin Secretion in Isolated Rat Pancreases*

摘要: This study demonstrates that the threshold of glucose-stimulated insulin secretion can be regulated in vivo by long term hormonal and nutrient modifications. The sensitivity pancreatic B-cell to glucose stimulation was determined examining pattern release from pancreases perfused with linear gradients. Male rats infused ovine PRL for 4 days receiving five hourly injections had a lower enhanced rates at all stimulatory concentrations. Infusion bGH without effect on gradient-stimulated release. Fasting 48 h resulted an elevation substantial reduction extent To determine possible processes involved these modifications secretion, vitro potentiators examined. Forskolin, glucagon, cholecystokinin, carbamylcholine ...