Uptake of Alkaloids by Latex Vesicles and Isolated Mesophyll Vacuoles of Chelidonium ntajus (Papaveraceae)

摘要: Latex vacuoles of Chelidonium majus (Papaveraceae) naturally sequester protoberberine and benzophenanthridine alkaloids. Isolated vesicles take up a number other heterologous alkaloids, such as 9,10-dihydroergocryptine, vinblastine, strychnine, nicotine, colchicine, lupanine, 13-hydroxylupanine, with strong preference for the indole alkaloids nicotine. Kinetic data clearly show that uptake proceeds via simple diffusion not by carrier-mediated transport. The are concentrated in against concentra­ tion gradient. MgATP has no influence on alkaloid uptake. It is likely trapped chelidonic acid (concentration latex 58 mM, 661 mM) which com plexes these thus prevents their rediffusion out vesicles. Uptake leaf protoplasts Ch. majus, suspension-cultured cells Lupinuspolyphyllus rabbit erythrocytes was studied comparison.