Measurement of delta pH and membrane potential in isolated neurosecretory vesicles from bovine neurohypophyses.
作者： J.T. Russell , R.W. Holz
DOI: 10.1016/S0021-9258(19)69106-4
关键词:
摘要: The steady state concentration ratios of [3H]methylamine and [14C]SCN- were measured to determine the pH gradient electrical potential, respectively, across membrane purified, intact, neurosecretory vesicles from bovine posterior pituitary. intravesicular was 5.8 when medium 7. Varying external had only a small effect on resulted in change gradient. Extrapolation zero yielded value 5.5 for pH. When 7, collapsed presence ammonium sulfate or combination nigericin K+. Measurement thiocyanate distribution vesicle suggested existence an inside negative potential. Mg2+ ATP caused marked shift potential +50 mV (inside positive) without altering This inhibited by carbonyl cyanide p-trifluoromethoxyphenylhydrazone. possible roles large storage peptide hormones their secretion is discussed.
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