A third essential DNA polymerase in S. cerevisiae.
作者： Alan Morrison , Hiroyuki Araki , Alan B. Clark , Robert K. Hamatake , Akio Sugino
DOI: 10.1016/0092-8674(90)90391-Q
关键词:
摘要: DNA polymerases I and III are essential for viability of S. cerevisiae. We have cloned analyzed POL2, the gene encoding catalytic subunit third nuclear polymerase, polymerase II. POL2 expressed a transcript approximately 7.5 kb contained reading frame that encoded protein calculated Mr 255,649. The N-terminal half predicted displayed relatively weak similarity sequence to eukaryotic polymerases. Disruption coding at midpoint led viable, slowly growing cells, which yielded truncated polypeptide with II activity, free from subunits B or C. Deletion resulted in inviability dumbbell terminal morphology typically follows arrest replication. conclude three yeast argue all replicases, possibility challenges existing models
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