Aptamer-Targeted Gold Nanoparticles As Molecular-Specific Contrast Agents for Reflectance Imaging
作者： David J. Javier , Nitin Nitin , Matthew Levy , Andrew Ellington , Rebecca Richards-Kortum
DOI: 10.1021/BC8001248
关键词:
摘要: Targeted metallic nanoparticles have shown potential as a platform for development of molecular-specific contrast agents. Aptamers recently been demonstrated ideal candidates molecular targeting applications. In this study, we investigated the aptamer-based gold agents, using aptamers agents and imaging We devised novel conjugation approach an extended aptamer design where extension is complementary to oligonucleotide sequence attached surface nanoparticles. The chemical optical properties aptamer-gold conjugates were characterized size measurements quantitation assays. demonstrate create agent designed detection prostate-specific membrane antigen (PSMA), obtaining reflectance images PSMA(+) PSMA(-) cell lines treated with anti-PSMA conjugates. This strategy can easily be modified incorporate multifunctional part multimodal
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