Use of nanoparticles for targeted, noninvasive thermal destruction of malignant cells.

摘要: Shortwave (MHz range) radiofrequency (RF) energy is nonionizing, penetrates deeply into biological tissues with no adverse side effects, and heats metallic nanoparticles efficiently. Targeted delivery of these to cancer cells should result in hyperthermic cytotoxicity upon exposure a focused, noninvasive RF field. We have demonstrated that gold conjugated cetuximab (C225) are quickly internalized by Panc-1 (pancreatic adenocarcinoma) Difi (colorectal overexpressing epidermal growth factor receptor (EGFR). or treated naked nonspecific IgG-conjugated minimal intracellular uptake transmission electron microscopy (TEM). In contrast, there were dense concentrations cytoplasmic vesicles containing following treatment cetuximab-conjugated nanoparticles. Exposure field produced nearly 100% the nanoparticles, but significantly lower levels two control groups (p < 0.00012). Treatment breast cell line (CAMA-1) does not express EGFR enhanced Conjugation cell-directed targeting agents may represent an effective cancer-specific therapy treat numerous types human malignant disease using hyperthermia.