Development and Characterization of a Largemouth Bass Cell Line
作者： Rodman G. Getchell , Geoffrey H. Groocock , Emily R. Cornwell , Vanessa L. Schumacher , Lindsay I. Glasner
DOI: 10.1080/08997659.2014.922517
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摘要: AbstractThe development and characterization of a new cell line, derived from the ovary Largemouth Bass Micropterus salmoides, is described. Gonad tissue was collected that were electrofished Oneida Lake, New York. The processed grown in culture flasks at approximately 22°C for more than 118 passages during an 8-year period 2004 to 2011. identity these cells as origin confirmed by sequencing portion cytochrome b gene. Growth rate three different temperatures documented. line susceptible virus (LMBV) its replication compared with Bluegill Lepomis macrochirus fry (BF-2), one lines recommended LMBV isolation American Fisheries Society Fish Health Section Blue Book. Quantitative PCR results trial showed BF-2 produced 10-fold copies per after 6 d, w...
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