Viral susceptibility, transfection and growth of SPB--a fish neural progenitor cell line from the brain of snubnose pompano, Trachinotus blochii (Lacépède).

作者: C-M Wen , C-C Ku , C-S Wang

DOI: 10.1111/JFD.12067

关键词:

摘要: This study investigates the susceptibilities of SPB cell line to fish viruses including giant seaperch iridovirus (GSIV-K1), red sea bream (RSIV-Ku), grouper nervous necrosis virus (GNNV-K1), chum salmon reovirus (CSV) and eel herpesvirus (HVA). GSIV-K1, RSIV-Ku CSV replicated well in cells, with a significant cytopathic effect production. However, cells were HVA GNNV refractory. To examine ability stably express foreign protein, expression vectors encoding B1 B2 fused enhanced green fluorescent protein (EGFP) GSIV ORF35L DsRed constructed introduced by transfection into cells. Stable transfectants displayed different morphologies compared each other. EGFP-B1 was predominantly localized nuclei, EFPF-B2 distributed throughout cytoplasm nucleus, granular 35L-DsRed secreted vesicles. The produced blebs on surface, but showing stable EGFP, or showed normal morphologies. Results show transfected grow at temperatures between 20 35 °C serum-dependent growth. are suitable for studies virology.

参考文章(67)
Jeong Wan Do, Chang Hoon Moon, Hyo Jeong Kim, Myoung Seok Ko, Sung Bum Kim, Ji Hee Son, Jong Soo Kim, Eun Jeong An, Min Kyoung Kim, Seung Koo Lee, Myung Shin Han, Seung Ju Cha, Mi Seon Park, Myoung Ae Park, Yi Cheong Kim, Jin Woo Kim, Jeong Woo Park, Complete genomic DNA sequence of rock bream iridovirus Virology. ,vol. 325, pp. 351- 363 ,(2004) , 10.1016/J.VIROL.2004.05.008
Jian G. He, Min Deng, Shao P. Weng, Zheng Li, Song Y. Zhou, Qin X. Long, Xun Z. Wang, Siu-Ming Chan, Complete genome analysis of the mandarin fish infectious spleen and kidney necrosis iridovirus. Virology. ,vol. 291, pp. 126- 139 ,(2001) , 10.1006/VIRO.2001.1208
Chuanfu Dong, Shaoping Weng, Xiujuan Shi, Xiaopeng Xu, Nan Shi, Jianguo He, Development of a mandarin fish Siniperca chuatsi fry cell line suitable for the study of infectious spleen and kidney necrosis virus (ISKNV). Virus Research. ,vol. 135, pp. 273- 281 ,(2008) , 10.1016/J.VIRUSRES.2008.04.004
SC Chi, JR Shieh, SJ Lin, Genetic and antigenic analysis of betanodaviruses isolated from aquatic organisms in Taiwan. Diseases of Aquatic Organisms. ,vol. 55, pp. 221- 228 ,(2003) , 10.3354/DAO055221
Xiaozhe Fu, Ningqiu Li, Lihui Liu, Qiang Lin, Fang Wang, Yingtiao Lai, Haiming Jiang, Houjun Pan, Cunbin Shi, Shuqin Wu, Genotype and host range analysis of infectious spleen and kidney necrosis virus (ISKNV) Virus Genes. ,vol. 42, pp. 97- 109 ,(2011) , 10.1007/S11262-010-0552-X
Malte Adelmann, Bernd Köllner, Sven. M. Bergmann, Uwe Fischer, Bodo Lange, Werner Weitschies, Peter-Joachim Enzmann, Dieter Fichtner, Development of an oral vaccine for immunisation of rainbow trout (Oncorhynchus mykiss) against viral haemorrhagic septicaemia. Vaccine. ,vol. 26, pp. 837- 844 ,(2008) , 10.1016/J.VACCINE.2007.11.065
SL Chen, GC Ren, ZX Sha, CY Shi, Establishment of a continuous embryonic cell line from Japanese flounder Paralichthys olivaceus for virus isolation. Diseases of Aquatic Organisms. ,vol. 60, pp. 241- 246 ,(2004) , 10.3354/DAO060241