The mechanism of interactions between tea polyphenols and porcine pancreatic alpha-amylase: Analysis by inhibition kinetics, fluorescence quenching, differential scanning calorimetry and isothermal titration calorimetry.

作者: Lijun Sun , Michael J. Gidley , Frederick J. Warren

DOI: 10.1002/MNFR.201700324

关键词:

摘要: Scope: This study aims to use a combination of biochemical and biophysical methods derive greater mechanistic understanding the interactions between tea polyphenols porcine pancreatic alpha-amylase (PPA).

参考文章(36)
Prakash Chandra Rathi, Karl-Erich Jaeger, Holger Gohlke, Structural Rigidity and Protein Thermostability in Variants of Lipase A from Bacillus subtilis PLOS ONE. ,vol. 10, pp. e0130289- ,(2015) , 10.1371/JOURNAL.PONE.0130289
F G Prendergast, J Lu, P J Callahan, Oxygen quenching of sensitized terbium luminescence in complexes of terbium with small organic ligands and proteins. Journal of Biological Chemistry. ,vol. 258, pp. 4075- 4078 ,(1983) , 10.1016/S0021-9258(18)32585-7
M Violet, J C Meunier, Kinetic study of the irreversible thermal denaturation of Bacillus licheniformis alpha-amylase. Biochemical Journal. ,vol. 263, pp. 665- 670 ,(1989) , 10.1042/BJ2630665
Gary Williamson, Possible effects of dietary polyphenols on sugar absorption and digestion. Molecular Nutrition & Food Research. ,vol. 57, pp. 48- 57 ,(2013) , 10.1002/MNFR.201200511
Madaisy Cueto, M Jesús Dorta, Obdulia Munguı́a, Matı́as Llabrés, New approach to stability assessment of protein solution formulations by differential scanning calorimetry. International Journal of Pharmaceutics. ,vol. 252, pp. 159- 166 ,(2003) , 10.1016/S0378-5173(02)00627-0
Meltem Yilmazer-Musa, Anneke M. Griffith, Alexander J. Michels, Erik Schneider, Balz Frei, Grape seed and tea extracts and catechin 3-gallates are potent inhibitors of α-amylase and α-glucosidase activity. Journal of Agricultural and Food Chemistry. ,vol. 60, pp. 8924- 8929 ,(2012) , 10.1021/JF301147N
Harshadrai M Rawel, Sascha Rohn, Hans-Peter Kruse, Jürgen Kroll, Structural changes induced in bovine serum albumin by covalent attachment of chlorogenic acid Food Chemistry. ,vol. 78, pp. 443- 455 ,(2002) , 10.1016/S0308-8146(02)00155-3
Mauno Vihinen, Relationship of protein flexibility to thermostability Protein Engineering. ,vol. 1, pp. 477- 480 ,(1987) , 10.1093/PROTEIN/1.6.477