Links between the Oncoprotein YB-1 and Small Non-Coding RNAs in Breast Cancer

摘要: Background: The nucleic acid-binding protein YB-1, a member of the cold-shock domain family, has been implicated in progression breast cancer and is associated with poor patient survival. YB-1 sequence similarity to LIN28, another family member, which role regulation small noncoding RNAs (sncRNAs) including microRNAs (miRNAs). Therefore, investigate whether there an association between sncRNAs cancer, we investigated were bound by two cell lines (luminal A-like basal cell-like), abundance mRNAs changed response experimental reduction expression. Results: RNA-immunoprecipitation anti-YB-1 antibody showed that several are YB-1. Some these both lines; others cell-line specific. derived from various sncRNA families miRNAs such as let-7 miR-320, transfer RNAs, ribosomal nucleolar (snoRNA). Reducing expression altered number transcripts encoding miRNA biogenesis processing proteins but did not alter mature or precursor miRNAs. Conclusions: binds specific miRNAs, snoRNAs tRNA-derived fragments appears regulate machinery. We propose some oncogenic effects may be mediated through its interactions sncRNAs.