Characterization of B lymphopoiesis in mouse bone marrow and spleen.

摘要: This chapter provides information on the application of flow cytometry for analysis B-cell development, describing in detail particular surface proteins that can serve as markers recognizing distinct stages this process. These cell fractions range from just prior to initial heavy chain rearrangement, germline pro-B stage, through D-J rearranged and expressing pre-B stages, maturing BCR positive stages. It also outlines assays characterization these cells, including procedures testing functional lineage restriction, determination rearrangement status, analyses gene expression at ribonucleic acid (RNA) protein level, assessment cycle state.