Murine epidermal growth factor (EGF) fragment (33-42) inhibits both EGF- and laminin-dependent endothelial cell motility and angiogenesis

摘要: Laminin, murine epidermal growth factor (mEGF), and the synthetic laminin peptide Lam.B1(925-933) (a linear from B1 chain of laminin, CDPGY1GSR-amide) all stimulate endothelial cell motility above basal rates, whereas a mEGF fragment, mEGF33-42 C-loop mEGF, acetyl-C-[S-Acm]-VIGYSGDR-C-[S-Acm]-amide), inhibits motility. In both human SK HEP-1 embryonic chick cells, blocks EGF- laminin-stimulated locomotion cells. vivo, also laminin- mEGF-induced angiogenesis in chick. line. has an additive effect coincubation with either or but it their effects alone stimulates laminin-induced angiogenesis. Thus, acts as general antagonist, agonist cells partial antagonist. We propose that presence anionic group at eighth residue may be source antagonistic seen this compared fragment. These findings have important implications design antiangiogenic agents, use models study disease.