The measurement of the main PGE metabolite, 13,14-dihydro-15-keto prostaglandin E by radioimmunoassay using methyl oxime stabilization.

摘要: Abstract The measurement of 13,14-dihydro-15-keto prostaglandin E 2 [PGEM] is complicated by the artefactual formation compounds corresponding A series which are reactive towards protein. Existing methods assay depend on deliberate dehydration to ‘A’ form followed cyclization in alkaline solution a bicyclic derivative stable and can be measured radioimmunoassay. We report an alternative approach using methyl oximation 9- 15-keto groups confer stability molecule. This derivatization simple does not involve active intermediate such as those PGA series. antiserum for radioimmunoassay raised against oxime form. label PGEM coupled tripeptide Pro-gly-tyr through nitrogen proline ring. linkage distinct from that used raise thus preferentially recognized over endogenous analyte; this results high sensitivity assay. correlated well with when both assays were measure same samples peripheral blood women receiving sustained release PGE pessary ripening cervix. technique provides rapid reliable method determining metabolites.