Radioimmunologic determination of 15-keto-13,14-dihydro-PGE2: a method for its stable degradation product, 11-deoxy-15-keto-13,14-dihydro-11 beta, 16 xi-cyclo-PGE2.
作者： Elisabeth Granström , F.A. Fitzpatrick , Hans Kindahl
DOI: 10.1016/0076-6879(82)86203-4
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摘要: Publisher Summary This chapter describes the chemical instability of 15-keto- 13,14-dihydro-PGE2 under various conditions, development a radioimmunoassay (RIA)for stable bicyclic degradation product, and conditions for induced quantitative conversion 15-ketodihydro-PGE2 in biological samples into this product prior to measurements. 15-ketodihydro-PGA2 undergo different fate cyclization l l-deoxy-15-keto-13,14-dihydro- 1 lβ,16ξ-cyclo-PGE2. contains at least two epimeric sites, C-8 C-16; furthermore, PGA2 derivative might also involve transient formation corresponding PGC2 compound, resulting additional sites C-11 C-12. The described is developed using racemic mixture these epimers preparation immunogen. crucial point RIA all present sample before analysis.
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