Effect of lead on fibroblasts taken from an 84-year-old donor

摘要: Abstract Human, diploid fibroblasts from an 84-year-old donor (A1Pos) were used to study the effect of 20, 40 and 80 μg/100 ml concentrations lead on in vitro senescence. The results showed that there was a significant decrease number total population doublings due treatment with lead. However, no difference between effects three As far as nucleolar is concerned case controls well lead-treated cells remained constant aging vitro. When areas dry mass cells, nuclei nucleoli examined groups at early late general changes noted exception which caused increase these parameters are discussed compared those previous [17] embryonic, IMR-90 treated similar way. It concluded A1Pos even more vulnerable than embryonic indicated by drastic curtailment lifespan exhibited lowest concentration overall lack change seen numbers area measurements result attributed fact nearer end their lifespan, had achieved cytological characteristics old therefore did now show much change.