Invariant Natural Killer T cells resilience to paradoxical sleep deprivation-associated stress

摘要: Abstract Although several studies demonstrate that stressful situations, such as sleep disturbances, negatively impact the innate and adaptive arms of immune system, their influence on invariant Natural Killer T (iNKT) cells remains unclear. iNKT are CD1d-restricted recognize glycolipid antigens rapidly produce polarizing cytokines being key players in responses, a potential target for immunotherapy. differ aspects from conventional lymphocytes, including unique dependence CD1d-expressing double-positive (DP) thymocytes intrathymic maturation. As consequence stress, DP undergo glucocorticoid-induced apoptosis, which might compromise developmental pathway. Therefore, we used paradoxical deprivation (SD) model to determine disturbance cell biology. After 72 h SD, C57Bl/6 mice exhibited significant increase systemic glucocorticoid levels thymus atrophy. Despite marked decrease number thymocytes, ratio CD1d+/CD1d− was higher SD mice, thymic remained unaltered, suggesting did not In contrast, reduced hepatic IFN-γ, but not, IL-4-producing cells, without further effect spleen. this fact, affect stimulation IFN-γ production by or cytokine release, response α-galactosylceramide, specific antigen. Furthermore, although impaired splenic NK activity against tumor it cell-specific cytotoxicity. Thus, our study shows SD-induced stress impair cells’ responses cognate