Parallel PRC2/cPRC1 and vPRC1 pathways silence lineage-specific genes and maintain self-renewal in mouse embryonic stem cells.
作者： J. A. Zepeda-Martinez , C. Pribitzer , J. Wang , D. Bsteh , S. Golumbeanu
关键词:
摘要: The transcriptional repressors Polycomb repressive complex 1 (PRC1) and PRC2 are required to maintain cell fate during embryonic development. PRC1 catalyze distinct histone modifications, establishing chromatin at shared targets. How PRC1, which consists of canonical (cPRC1) variant (vPRC1) complexes, cooperate silence genes support mouse stem (mESC) self-renewal is unclear. Using combinatorial genetic perturbations, we show that independent pathways cPRC1 vPRC1 responsible for maintenance H2A monoubiquitylation silencing target genes. Individual loss PRC2-dependent or PRC2-independent disrupts only one pathway does not impair mESC capacity. However, both leads differentiation activation a subset lineage-specific co-occupied by relatively high levels PRC1/PRC2. Thus, parallel explain the differential requirements provide robust
sciencemag.org
harvard.edu参考文章(61)
K. Hisada, C. Sanchez, T. A. Endo, M. Endoh, M. Roman-Trufero, J. Sharif, H. Koseki, M. Vidal, RYBP Represses Endogenous Retroviruses and Preimplantation- and Germ Line-Specific Genes in Mouse Embryonic Stem Cells Molecular and Cellular Biology. ,vol. 32, pp. 1139- 1149 ,(2012) , 10.1128/MCB.06441-11
Ragnhild Eskeland, Martin Leeb, Graeme R. Grimes, Clémence Kress, Shelagh Boyle, Duncan Sproul, Nick Gilbert, Yuhong Fan, Arthur I. Skoultchi, Anton Wutz, Wendy A. Bickmore, Ring1B Compacts Chromatin Structure and Represses Gene Expression Independent of Histone Ubiquitination Molecular Cell. ,vol. 38, pp. 452- 464 ,(2010) , 10.1016/J.MOLCEL.2010.02.032
Jason Wray, Tüzer Kalkan, Sandra Gomez-Lopez, Dominik Eckardt, Andrew Cook, Rolf Kemler, Austin Smith, Inhibition of glycogen synthase kinase-3 alleviates Tcf3 repression of the pluripotency network and increases embryonic stem cell resistance to differentiation Nature Cell Biology. ,vol. 13, pp. 838- 845 ,(2011) , 10.1038/NCB2267
R. Cao, L. Wang, H. Wang, L. Xia, H. Erdjument-Bromage, P. Tempst, R. S. Jones, Y. Zhang, Role of Histone H3 Lysine 27 Methylation in Polycomb-Group Silencing Science. ,vol. 298, pp. 1039- 1043 ,(2002) , 10.1126/SCIENCE.1076997
Lluis Morey, Gloria Pascual, Luca Cozzuto, Guglielmo Roma, Anton Wutz, Salvador Aznar Benitah, Luciano Di Croce, Nonoverlapping Functions of the Polycomb Group Cbx Family of Proteins in Embryonic Stem Cells Cell Stem Cell. ,vol. 10, pp. 47- 62 ,(2012) , 10.1016/J.STEM.2011.12.006
P. Voigt, W.-W. Tee, D. Reinberg, A double take on bivalent promoters Genes & Development. ,vol. 27, pp. 1318- 1338 ,(2013) , 10.1101/GAD.219626.113
Zhonghua Gao, Jin Zhang, Roberto Bonasio, Francesco Strino, Ayana Sawai, Fabio Parisi, Yuval Kluger, Danny Reinberg, PCGF homologs, CBX proteins, and RYBP define functionally distinct PRC1 family complexes. Molecular Cell. ,vol. 45, pp. 344- 356 ,(2012) , 10.1016/J.MOLCEL.2012.01.002
Reinhard Kalb, Sebastian Latwiel, H Irem Baymaz, Pascal W T C Jansen, Christoph W Müller, Michiel Vermeulen, Jürg Müller, Histone H2A monoubiquitination promotes histone H3 methylation in Polycomb repression. Nature Structural & Molecular Biology. ,vol. 21, pp. 569- 571 ,(2014) , 10.1038/NSMB.2833
Mariana de Napoles, Jacqueline E Mermoud, Rika Wakao, Y Amy Tang, Mitusuhiro Endoh, Ruth Appanah, Tatyana B Nesterova, Jose Silva, Arie P Otte, Miguel Vidal, Haruhiko Koseki, Neil Brockdorff, None, Polycomb Group Proteins Ring1A/B Link Ubiquitylation of Histone H2A to Heritable Gene Silencing and X Inactivation Developmental Cell. ,vol. 7, pp. 663- 676 ,(2004) , 10.1016/J.DEVCEL.2004.10.005
M. Leeb, D. Pasini, M. Novatchkova, M. Jaritz, K. Helin, A. Wutz, Polycomb complexes act redundantly to repress genomic repeats and genes Genes & Development. ,vol. 24, pp. 265- 276 ,(2010) , 10.1101/GAD.544410