Characterization of the staphylococcal bacteriophage lysin CHAPK

摘要: Aims:  To develop an efficient purification strategy for the bacteriophage lysin CHAPK. evaluate its antibacterial spectrum, enzymatic properties, optimal reaction conditions and lytic activity against live Staphlyococcus aureus. Methods Results:  Recombinant CHAPK was purified to homogeneity by cation exchange chromatography, with yields of up 10 mg from 1 l Escherichia coli culture. The spectrum includes all staphylococcal species also members genera Micrcococcus, Streptococcus, Nesterenkonia, Arthrobacter, Leuconostoc Carnobacterium. enzyme active pH 6 11 optimum at 9, 5 40°C, 15°C. When cell lysis lysostaphin compared over a concentration range 2·5–10 μg ml−1 using Staph. aureus 5 min 37°C, gave rise greater turbidity reduction indicating that it works more rapidly than lysostaphin. Conclusions:  This study describes in detail characteristics novel phage-derived demonstrating has excellent biochemical properties as anti-staphylococcal agent. Significance Impact Study:  Currently, there is need new antimicrobial agents due increasing worldwide prevalence antibiotic resistance. Our findings demonstrate potential development alternative therapeutic pathogenic staphylococci including MRSA.