Role of superoxide in apoptosis induced by growth factor withdrawal
作者： Wilfred Lieberthal , Veronica Triaca , Jason S. Koh , Patrick J. Pagano , Jerrold S. Levine
DOI: 10.1152/AJPRENAL.1998.275.5.F691
关键词:
摘要: We have examined the role of reactive oxygen species (ROS) in apoptosis induced by growth factor deprivation primary cultures mouse proximal tubular (MPT) cells. When confluent monolayers MPT cells are deprived all factors, die over a 10- and 14-day period. Both epidermal (EGF) high-dose insulin directly inhibit factors. Growth results an increase cellular levels superoxide anion while withdrawal is inhibited number antioxidants scavengers ROS. also activation caspase activity, which EGF as well ROS that apoptosis. The cell-permeant inhibitor, z-Val-Ala-Asp-CH2F (zVAD-fmk), prevents activity markedly inhibits deprivation. However, zVAD-fmk had no effect on increased associated with Thus we provide novel evidence play important mediating appear to act upstream caspases apoptotic pathway. hypothesize oxidant stress, withdrawal, represents signaling mechanism for default pathway
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