Laser Time-Resolved Fluorescence Studies for Investigating Chromophore-Biosubstrate Interactions

摘要: Fluorimetric studies utilizing continuous wave (cw) techniques performed on chromophores that bind specifically to the biomacromolecule or cell organelle compartment be examined allowed a great deal of information gained, in past, biomolecular structures and kinetics (Udenfried, 1969). Flow cytometry, which has become such an important methodology for auto mated cytology, is just example extent fluorescence properties Acridines, ie. dyes exhibiting selective affinity nucleic acids (Albert, 1966), have been exploited determine, instance, DNA RNA contents single cells chromosomes aberrations cell-cycle phases (Mullaney et al., 1974). These investigations basically take advantage fact optical - fluorescence, particular are affected unequivocally by binding biomacromolecules biostruc- tures (Andreoni, 1985). Moreover, stronger and/or more disruptive interaction with substrate, pronounced is, usually, effect chromophore emission. This property so general variations observed both “in vitro” vivo” quantum yield emission spectrum fluorescent drugs commonly considered indicative stability their complexation (Geacintov, 1987) specific substances sites (Goormaghtigh 1980; Kessel 1985)