PARP-1/2 Inhibitor Olaparib Prevents or Partially Reverts EMT Induced by TGF-β in NMuMG Cells.
作者： Michelle Schacke , Janani Kumar , Nicholas Colwell , Kole Hermanson , Gustavo Folle
DOI: 10.3390/IJMS20030518
关键词:
摘要: Poly- adenosine diphosphate (ADP)-ribose (PAR) is a polymer synthesized as posttranslational modification by some poly (ADP-ribose) polymerases (PARPs), namely PARP-1, PARP-2, tankyrase-1, and tankyrase-2 (TNKS-1/2). PARP-1 nuclear has also been detected in extracellular vesicles. PARP-2 TNKS-1/2 are distributed nuclei cytoplasm. PARP or PAR alterations have described tumors, particular influencing the Epithelial- Mesenchymal Transition (EMT), which influences cell migration drug resistance cancer cells. Pro-EMT anti-EMT effects of reported while whether changes occur specifically during EMT currently unknown. The PARP-1/2 inhibitor Olaparib (OLA) approved FDA to treat certain patients harboring cancers with impaired homologous recombination. Here, we studied OLA on EMT. Total increased belts were disassembled. prevented EMT, according to: (i) molecular markers evaluated immuno-cytofluorescence/image quantification, Western blots, RNA quantitation, (ii) morphological expressed anisotropy, (iii) capacity scratch assay. partially reversed might work through unconventional mechanisms action (different from synthetic lethality), even non-BRCA (breast 1 gene) mutated cancers.
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