Separating the contributions of zona pellucida and cytoplasm in the viscoelastic response of human oocytes.

摘要: Abstract The successful characterization of the mechanical properties human oocytes and young embryos is crucial relevance to reduce risk pregnancy arrest in in-vitro fertilization processes. Unfortunately, current study has been hindered by lack accuracy describing contributions each structure (zona pellucida, cytoplasm) due its high heterogeneity. In this work, we present a novel approach model oocyte response taking into account effect both zona cytoplasm, as well different loading conditions. then applied develop an experimental protocol capable accurately separating viscoelastic contribution cytoplasm simply varying condition. This new potential open door improving our understanding at stages, provide quantitative predictive ability evaluation quality. Statement Significance Assisted reproductive technologies, such vitro fertilization, often rely on identifying quality or female egg cells. these cells, stiffness stress relaxation time, have identified objective indicators cell However, their proven difficult structural heterogeneity inconsistent paper presents that, although simple, addresses above difficulties accurate estimations cell’s properties. Learning from model, propose non-invasive testing allow with increased accuracy. We believe effort would improve consistency measurements enhance knowledge mechanics oocytes.