Low Expression of IL-10 in Circulating Bregs and Inverted IL-10/TNF-α Ratio in Tears of Patients With Perennial Allergic Conjunctivitis: A Preliminary Study

摘要: Allergic conjunctivitis (AC) is one of the most common ophthalmological disorders seen in clinical practice. Growing evidence from recent years suggests that a subset IL-10-expressing B cells involved inflammatory allergic diseases. In this study, we aimed to evaluate potential involvement blood Bregs perennial (PAC), and interleukins (IL)-1β, IL-6, IL-8, IL-10, IL-12, tumor necrosis factor (TNF)-α, were measured tear samples compared with healthy controls (HC) using flow cytometry. Non-significant differences CD19+IL-10+ cell frequency between PAC patients observed. Nevertheless, when analyzed mean fluorescence intensity (MFI) IL-10 on CD19+CD38Lo/Med/Hi-gated cells, observed significant decrease MFI all subsets patients. Additionally, cytokines showed 2.8 times lower levels than TNF-α HC. Our findings demonstrate an immunological dysregulation conjunctivitis, characterized by low expression circulating CD19+CD38+ inverted IL-10/TNF-α ratio, promoting local pro-inflammatory microenvironment. These highlight novel pathologic changes ocular Understanding systemic mechanisms will aid design immunomodulating therapeutics at different levels.