Serotonin and Histamine Therapy Increases Tetanic Forces of Myoblasts, Reduces Muscle Injury, and Improves Grip Strength Performance of Dmdmdx Mice

摘要: Duchenne muscular dystrophy (DMD) is a recessive X-linked fatal disorder caused by mutation in the dystrophin gene. Although several therapeutic approaches have been studied, none has led to substantial long-term effects patients. The aim of this study was test serotonin and histamine (S&H) combination on human skeletal myoblasts Dmd(mdx) mice for its muscle strength injury. Normal bioartificial muscles (BAMs) were treated, tetanic forces injury tests performed using MyoForce Analysis System. mice, murine model DMD, administered serotonin, histamine, or S&H twice daily 6 weeks, functional performance conducted once week. treatment significant increases at all time points concentrations tested as compared saline controls. Dose response BAMs demonstrated increase force generation controls after 3 4 days drug treatment. highest had effect lowering contractile-induced measured reduction release adenylate kinase. Histamine-only treatments improved grip whereas serotonin-only resulted no improvement strength. results indicate that therapy might be promising new strategy dystrophies mechanism should further investigated.