Quercetin protects embryonic chicken spermatogonial cells from oxidative damage intoxicated with 3-methyl-4-nitrophenol in primary culture

摘要: Abstract Diesel exhaust particles (DEP) are considered to be one of the most important air pollutants. In this study, protective effect quercetin, an antioxidant flavonoid, on oxidative damage testicular cells was studied by analysis intracellular system embryonic chickens after treatment with 3-methyl-4-nitrophenol (PNMC) derived from DEP. Testicular 18-day-old embryos were cultured in serum-free McCoys’5A medium and challenged PNMC (10−7 10−5 M) alone or combinations quercetin (1.0 μg/ml) for 48 h. Results showed that exposure (10−5 M) induced condensed nuclei vacuolated cytoplasm, a decrease cell viability spermatogonial number. Exposure lipid peroxidation elevation thiobarbituric acid reactive substances as well decreasing glutathione activity superoxide dismutase activity. However, simultaneous supplementation restored these parameters similar levels control. is therefore concluded have stress cells, which can attenuated combined treatment. Our results support therapeutic use prevention reproductive toxicity environmental toxicant PNMC.