Integration of Simple Sequence Repeat DNA Markers into a Soybean Linkage Map

摘要: A total of 40 simple sequence repeat (SSR) or microsatellite DNA markers were mapped in a soybean [Glycine max (L.) Merrill] mapping population that consisted 60 F 2 plants from cross between near isogenic lines the cultivars Clark and Harosoy. The first objective study was to determine map location SSR loci relation 13 classical controlling pigmentation morphological traits, seven isozyme loci, 118 RFLP RAPD markers. second if randomly distributed genome. Linkage analysis with MAPMAKER 3.0b yielded 29 linkage groups length 1486 centimorgans (cM). This compares 1056 cM removed data set. Thirty-four placed groups. linked nine two loci. Eighteen contained at least one locus. While this result suggested throughout genome, clusters five four spanning 23.4 33.6 cM, respectively, detected. These results indicated relatively limited amount clustering demonstrated genetic should provide an excellent complement for use molecular biology, genetics, breeding research. Because detect only single are highly polymorphic, they can be extremely informative pedigree tracing studies, progeny multiparent matings, wide range applications, genotype identification.