Large-scale preparation of synaptosomes from bovine brain using a zonal rotor technique.

摘要: A zonal rotor technique for the preparation of synaptosomes in bulk from bovine brain frontal cortex based on an empirical transformation a small-volume discontinuous surcrose density gradient arrangement is presented detail. The procedure yields new information concerning prepared sucrose gradients. Cerebroside analysis and electron microscopy show myelin contamination to be restricted leading, less dense edge synaptosomal profile, free mitochondria trailing, more edge. Exclusion fringe areas highly purified synaptosome which entirely enters next layer beyond 0.8∶1.2 M interface. This interface collects most oubain-sensitive (Na+, K+) adenosine triphosphatase activity. display very high intrinsic sialidase activity are rich di-, tri-, tetrasialogangliosides, preferred substrates enzyme. Up 90% cholinesterase specific acetylcholinesterase.