Principles for the Optimization of Centrifugation Conditions for Fractionation of Brain Tissue
作者： Carl W. Cotman
DOI: 10.1007/978-1-4615-7748-5_4
关键词: Sedimentation 、 Fractionation 、 Buoyant density 、 Brain tissue 、 Differential centrifugation 、 Chromatography 、 Chemistry 、 Centrifugation 、 Isopycnic 、 Rate-zonal centrifugation
摘要: Classes of subcellular particles separate by centrifugation procedures because significant differences in their sedimentation rates and buoyant densities. Most devised for brain make use both rate density. Preliminary enrichment is achieved differential centrifugation, usually angle rotors, followed separation density-on density gradients zonal or conventional rotors. Particles they differ size, density, shape. on (isopycnic centrifugation) prior to isopycnic banding (rate centrifugation).
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