作者: Pei-Zhong Tang , Phillip Gray , Thomas Chappell
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摘要: Compositions and methods are provided for generating three frame cDNA expression libraries functional screening of proteins. The invention includes sets 5' adapters cloning molecules in which the include that can be used to clone a particular all reading frames. so generated have greater complexity expressed open frames, thus improve success protein screens, such as two hybrid screens. also recombination sites efficient transfer between systems.