Method of expression cloning

摘要: The present invention provides improvements in the expression cloning method for isolating novel cDNA clones. In particular, comprising steps of preparing a library from cell that expresses desired protein; inserting into an vector; bacterial cells and culturing to produce individual colonies; collecting pools predetermined number cDNAs contained pools; expressing proteins encoded by cDNAs; detecting protein, positive clones may be efficiently cost-effectively obtained about 100 colonies vitro.