Photocurrents generated by bacteriorhodopsin adsorbed on nano-black lipid membranes.
作者： Christian Horn , Claudia Steinem
DOI: 10.1529/BIOPHYSJ.105.059550
关键词:
摘要: Purple membranes were adsorbed on freestanding lipid bilayers, termed nano-black (nano-BLMs), suspending the pores of porous alumina substrates with average pore diameters 280 nm. Nano-BLMs obtained by first coating upper surface highly ordered a thin gold layer followed chemisorption 1,2-dipalmitoyl-sn-glycero-3-phosphothioethanol and subsequent addition droplet 1,2-diphytanoyl-sn-glycero-3-phosphocholine octadecylamine dissolved in n-decane onto hydrophobic submonolayer. By means impedance spectroscopy, quality nano-BLMs was verified. The electrical parameters confirm formation single bilayers high membrane resistances covering matrix. Adsorption purple recording photocurrents generated bacteriorhodopsin upon continuous light illumination. system exhibits very long-term stability advantage that not only transient but also stationary currents are recordable. adding proton ionophore carbonyl cyanide-m-chlorophenylhydrazone conductivity increases, resulting higher current, which proves conductance occurs across nano-BLMs.
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