Nondestructive sampling of insect DNA from defensive secretion.

摘要: Nondestructive techniques to obtain DNA from organisms can further genetic analyses such as estimating diversity, dispersal and lifetime fitness, without permanently removing individuals the population or body parts. Possible sources for insects include frass, exuviae, wing leg clippings. However, these are not feasible approaches that cannot be removed their natural environment long periods when adverse effects of tissue removal must avoided. This study evaluated impacts efficacy extracting haemolymph a defensive secretion amplification microsatellites using nondestructive technique. A containing was obtained Bolitotherus cornutus (the forked fungus beetle) by perturbation gland with capillary tube. laboratory experiment demonstrated sampling methodology had no impact on mortality, reproductive success expression. To evaluate quality in samples, collected 187 field successfully genotyped at nine microsatellite loci 95.7% samples. These results indicate haemolymph-rich secretions contain sampled negative health fitness individual insects.