Development of a novel monoclonal antibody against 4-hydroxy-2E,6Z-dodecadienal (4-HDDE)-protein adducts: Immunochemical application in quantitative and qualitative analyses of lipid peroxidation in vitro and ex vivo.

摘要: Non-enzymatic peroxidation of polyunsaturated fatty acids (PUFA) results in the formation various α,β-unsaturated aldehydes, which 4-hydroxyalkenals are abundant. The propensity n-6 PUFA, such as linoleic acid, γ-linolenic acid and arachidonic to undergo radical-induced generate 4-hydroxy-2E-nonenal (4-HNE) has been widely demonstrated. ability latter form covalent adducts with macromolecules modify cellular functions linked numerous pathological processes. Concomitantly, evidence accumulated on specific signaling properties low concentrations 4-HNE that may induce hormetic protective responses stress cells. It long known particularly also give rise 4-hydroxy-2E,6Z-dodecadienal (4-HDDE), is more chemically reactive than 4-HNE. Few studies 4-HDDE revealed its avidly interact covalently electronegative moieties selectively activate transcriptional regulator Peroxisome Proliferator-Activated Receptor (PPAR)-β/δ. research impeded due lack available pure antibodies recognize 4-HDDE-modified epitopes proteins. purpose this study was employ an established procedure synthesize use it create characterize a monoclonal antibody against proteins establish application for ELISA immunohistochemical analysis cells tissues further expand lipid research.