Downstream Processing of Transgenic Plant Systems: Protein Recovery and Purification Strategies

摘要: Plant-derived recombinant proteins provide alternatives to produced by mammalian, microbial, and insect cell cultures due significant upstream technological achievements over the past 5–10 years. Plants offer flexibility in both growth methods (open-field, greenhouse, bioreactor) host expression systems (seeds, leaves, culture). The diversity of plant production provides for numerous commercial applications plant-derived but economic viability must be ensured through high levels scalable manufacturing processes. Initial research efforts biotechnology were focused on strategies and, thus, have not matched downstream processing advancements. However, case-by-case extraction studies led development system-based approaches. Other progress includes pretreatment improve purification efficiency reduce costs from green tissue homogenates that contain chlorophyll, phenolics, active enzymes. In spite all positive developments made last 10 years, continual breakthroughs are needed capitalize lower cost transgenic biomass. This chapter describes general advantages disadvantages seed-, leaf-, bioreactor-based used primary recovery proteins.