Epigenetic repression of regulator of G-protein signaling 2 by ubiquitin-like with PHD and ring-finger domain 1 promotes bladder cancer progression

摘要: Ubiquitin-like with PHD and ring-finger domain 1 (UHRF1) binds to methylated promoters of tumor-suppressor genes suppresses gene expression by forming complexes DNA methyltransferases. Recent studies have shown that repression regulator G-protein signaling (RGS) 2 increases cancer cell growth. However, little is known about whether UHRF1 promotes bladder progression epigenetic silencing RGS2. Here, we show increased in lines most tissues as compared normal controls. overexpression proliferation, whereas inhibition proliferation. In cells, inhibits RGS2 increasing the methylation CpG nucleotides promoter. analysis showed tumor-specific TGS2 promoter 73% (38/52) tumors. High correlated aberrant tumors, which results loss expression, confirmed demethylation lines. Functionally, re-expression partly abrogates UHRF1-induced Furthermore, Kaplan–Meier low significantly reduced overall survival patients cancer. These demonstrate contributes progression.