Detection of MGMT methylation in tumors

作者: Luca Morandi

DOI:

关键词:

摘要: A method for quantifying the methylated allele of 06-methylguanine-DNA methyltransferase (MGMT) promoter DNA in cancer cells, comprising: assaying MGMT formalin fixed paraffin embedded (FFPE) by methylation sensitive quantitative PCR.

参考文章(54)
David G. Ginzinger, Dan H. Moore, Tony E. Godfrey, Joe W. Gray, Maria G. Pallavicini, Ronald H. Jensen, Janice Nigro, Seiji Suzuki, Measurement of DNA copy number at microsatellite loci using quantitative PCR analysis. Cancer Research. ,vol. 60, pp. 5405- 5409 ,(2000)
Osman El-Maarri, Karin Buiting, Edwin G Peery, Peter M Kroisel, Basak Balaban, Klaus Wagner, Bulent Urman, Julia Heyd, Christina Lich, Camilynn I Brannan, Jörn Walter, Bernhard Horsthemke, None, Maternal methylation imprints on human chromosome 15 are established during or after fertilization Nature Genetics. ,vol. 27, pp. 341- 344 ,(2001) , 10.1038/85927
Xilin C. Qian, Thomas P. Brent, Methylation Hot Spots in the 5′ Flanking Region Denote Silencing of the O6-Methylguanine-DNA Methyltransferase Gene Cancer Research. ,vol. 57, pp. 3672- 3677 ,(1997)
Jean-Pierre Issa, CpG island methylator phenotype in cancer Nature Reviews Cancer. ,vol. 4, pp. 988- 993 ,(2004) , 10.1038/NRC1507
Jörg Tost, Jenny Dunker, Ivo Glynne Gut, Analysis and quantification of multiple methylation variable positions in CpG islands by Pyrosequencing. BioTechniques. ,vol. 35, pp. 152- 156 ,(2003) , 10.2144/03351MD02
Jeannie Kibitel, Kurt Jaeckle, Daniel Yarosh, Michael Belanich, Harmon Eyre, Stephen Coons, Patricia Wasserman, Michael Prados, William Shapiro, Lori Alas, Denise Guerra, Monica Pastor, Ben Li, Dori Rector, Agnes White, Marc Citron, Susan Schulman, Terri Randall, Retrospective study of the correlation between the DNA repair protein alkyltransferase and survival of brain tumor patients treated with carmustine Cancer Research. ,vol. 56, pp. 783- 788 ,(1996)
Lili Liu, Sanford Markowitz, Stanton L. Gerson, Mismatch repair mutations override alkyltransferase in conferring resistance to temozolomide but not to 1,3-bis(2-chloroethyl)nitrosourea. Cancer Research. ,vol. 56, pp. 5375- 5379 ,(1996)