Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge

摘要: In various embodiments methods of determining methylation DNA are provided. one illustrative, but non-limiting embodiment the method comprises i) contacting a biological sample comprising nucleic acid to first matrix material column or filter where said binds and/or filters acids in and thereby purifies DNA; ii) eluting bound from denaturing produce eluted denatured iii) heating presence bisulfite ions deaminated acid; iv) second bind material; v) desulfonating simultaneously by with an alkaline solution converted vi) vii) performing specific PCR sequencing, high resolution melting analysis (HRM) on bisulfite-converted determine acid, wherein at least steps through performed single reaction cartridge.