Brief report: Dclk1 deletion in tuft cells results in impaired epithelial repair after radiation injury.

摘要: The role of Dclk1+ tuft cells in the replacement intestinal epithelia and reestablishing epithelial barrier after severe genotoxic insult is completely unknown. Successful restoration requires precise coordination between within each crypt subunit. While mechanisms that control this response remain largely uncertain, radiation model remains an exceptional surrogate for stem cell-associated loss. Following creation Dclk1-intestinal-epithelial-deficient Villin-Cre;Dclk1flox/flox mice, widespread gene expression changes were detected isolated during homeostasis. number surviving crypts was unaffected, mice failed to maintain tight junctions died at approximately 5 days, where Dclk1flox/flox lived until day 10 following injury. These findings suggest Dclk1 plays a functional critical restorative response. Stem Cells 2014;32:822–827