Survival of Patients with Gastrointestinal Cancers Can Be Predicted by a Surrogate microRNA Signature for Cancer Stem–like Cells Marked by DCLK1 Kinase

摘要: Doublecortin-like kinase 1 (DCLK1) is a gastrointestinal (GI) tuft cell that has been investigated as biomarker of cancer stem-like cells in colon and pancreatic cancers. However, its utility may be limited principle by signal instability dilution heterogeneous tumors, where the proliferation diverse tumor lineages obscures direct measurement DCLK1 activity. To address this issue, we explored definition miRNA signature surrogate for cells. Utilizing RNA/miRNA-sequencing datasets from Cancer Genome Atlas, identified 15-miRNA expression activity across several GI cancers, including colon, pancreatic, stomach Notably, Cox regression Kaplan-Meier analysis demonstrated could predict survival patients with these Moreover, patient subgroups predicted clinical biomarker. Our findings greatly strengthen significance Further, they provide an initial guidepost toward development improved prognostic biomarkers or companion DCLK1-targeted therapies to eradicate malignancies. Res; 76(14); 4090-9. ©2016 AACR.