Overexpression of PGA37/MYB118 and MYB115 promotes vegetative-to-embryonic transition in Arabidopsis
作者： Xingchun Wang , Qi-Wen Niu , Chong Teng , Chao Li , Jinye Mu
DOI: 10.1038/CR.2008.276
关键词:
摘要: Formation of somatic embryos from non-germline cells is unique to higher plants and can be manipulated in a variety species. Previous studies revealed that overexpression several Arabidopsis genes, including WUSCHEL (WUS)/PLANT GROWTH ACTIVATOR6 (PGA6), BABY BOOM, LEAFY COTYLEDON1 (LEC1), LEC2, able cause vegetative-to-embryonic transition or the formation embryos. Here, we report gain-of-function mutation PGA37 gene, encoding MYB118 transcription factor, induced transition, root explants, an elevated LEC1 expression level. Double mutant analysis showed WUS was not required for induction by PGA37/MYB118. In addition, MYB115, homolog PGA37/MYB118, caused pga37-like phenotype. A myb118 myb115 double did show apparent developmental abnormalities. Collectively, these results suggest PGA37/MYB118 MYB115 promote through signaling pathway independent WUS.
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