Acyl-CoA oxidase 1 is involved in γ-decalactone release from peach (Prunus persica) fruit.
作者： Liping Zhang , Haiyan Li , Ling Gao , Yujie Qi , Wanyi Fu
DOI: 10.1007/S00299-017-2113-4
关键词:
摘要: KEY MESSAGE γ-Decalactone accumulation in peach mesocarp was highly correlated with ACX enzyme activity and natural PpACX1 content. Adding the purified recombinant induced γ-decalactone biosynthesis cultured discs vitro. Previous gene expression studies have implied that acyl coenzyme A oxidase (ACX) is related to lactones synthesis, characteristic aroma compounds of peach. Here, we analysed correlation between content five different types fully ripe varieties. Furthermore, 'Hu Jing Mi Lu' ('HJ') 'Feng Hua Yu ('YL'), which strong among them, at four ripening stages were selected study role lactone biosynthesis. The result showed most abundant compound. activity. Mass spectrometry (MS) PpACX protein cv. 'HJ'. To further elucidate function protein, heterologously expressed a bacterial system characterized MS identification gave molecular weight as 94.44 kDa coverage rate peptide segments 47.3%. In vitro, adding C16-CoA substrate expected Using sandwich ELISA based on mixed mono- polyclonal antibodies against PpACX1, found be varieties 'HJ' 'YL'. This revealed vital fruit.
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