Identification of volatile and softening-related genes using digital gene expression profiles in melting peach

摘要: To reveal the comprehensive mechanism which is associated with biosynthesis of volatile compounds and accompanying texture change, RNA-seq was employed to survey differentially expressed genes (DEGs), at transcriptional level, one cultivar three stages ripening four melting peach cultivars harvest stage. Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analysis showed that highly ranked are involved in “Ribosome” “Plant-pathogen interaction,” “Flavonoid biosynthesis,” “Linoleic acid metabolism,” “Flavone flavonol biosynthesis” during fruit-ripening process. The quantitative real-time PCR (qRT-PCR) validation 15 aroma softening-related high correlation results. Transcripts a nonspecific lipid transfer protein (nsLTP, ppa013554) an ATP-binding cassette transporter (ABC, ppa002351) increased from early stage commercial then declined fully highest transcript abundance aroma-enriched cv. “Hu Jing Mi Lu” (HJ) lowest “Zhong Hua Shou Tao” slight aroma. Fifty gene families related formation were found. For lactone biosynthesis, we inferred ppa002510 ppa002282 may be two important acyl-CoA oxidase correlating difference γ-decalactone concentrations among cultivars. expression 3-hydroxyacyl-CoA dehydrogenase (HCAD, ppa008854) upregulated ripening. Thirteen fruit softening. Four aquaporin (AQP) cultivar-specificity for HJ, them, ppa009506, reached maximum accumulation transcripts batch novel ACX, AOC, ABC, HCAD, AQP) found here facilitates understanding molecular