Comparative analysis of expressed sequence tags from tissues in ripening stages of peach (Prunus persica L. Batsch)

摘要: Expressed sequence tag (EST) represents a resource for gene discovery, genome annotation and comparative genomics in plants. ESTs were derived by sequencing clones from five libraries created two different fruit tissues (skin mesocarp), at four ripening stages (from post-allegation to post-climacteric) three genotypes of peach (OroA, Bolero Suncrest). A total 10,847 EST sequences produced (dataset A); addition, 21,857 B) obtained public databases. Clustering assembly both datasets gave 17,858 unigenes. Analysis the allowed assignment putative function 70.8% ESTs. In order define relationship among transcriptome, ontology analysis was performed. Differences organs maturation same identified organelle, signal transducer antioxidant activity. distance matrix pairwise correlation coefficients applied between libraries. Shoot appeared outgroup our proved be an efficient tool parallel complement expression studies (for example, based on microarray analysis). We conducted frequency genes putatively involved metabolism some volatiles, which pointed predominant presence those transcripts skin. The metabolic pathways esters lactones selected further isolation cloning key genes. database is available web site www.itb.cnr.it/estree.