Direct determination of single-to-double stranded DNA ratio in solution using steady-state fluorescence measurements
作者： Lori Beach , Claude Schweitzer , J. C. Scaiano
DOI: 10.1039/B209284K
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摘要: We report a simple and rapid method for quantitation of single-to-double stranded (ss : ds) DNA ratios in solution, using steady-state measurements fluorescence from two simultaneously excited intercalated dyes; the ratio intensities PicoGreen (525 nm) ethidium bromide (610 is directly proportional to ss ds ratio.
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sci-hub.se 参考文章(4)
G. Cosa, K.-S. Focsaneanu, J. R. N. McLean, J. P. McNamee, J. C. Scaiano, Photophysical Properties of Fluorescent DNA‐dyes Bound to Single‐ and Double‐stranded DNA in Aqueous Buffered Solution¶ Photochemistry and Photobiology. ,vol. 73, pp. 585- 599 ,(2001) , 10.1562/0031-8655(2001)073<0585:PPOFDD>2.0.CO;2
Victoria L. Singer, Laurie J. Jones, Stephen T. Yue, Richard P. Haugland, Characterization of PicoGreen Reagent and Development of a Fluorescence-Based Solution Assay for Double-Stranded DNA Quantitation Analytical Biochemistry. ,vol. 249, pp. 228- 238 ,(1997) , 10.1006/ABIO.1997.2177
Kim R. Rogers, Alma Apostol, Steen J. Madsen, Charles W. Spencer, Detection of low dose radiation induced DNA damage using temperature differential fluorescence assay. Analytical Chemistry. ,vol. 71, pp. 4423- 4426 ,(1999) , 10.1021/AC990537Z
Eckhard Nordmeier, Absorption spectroscopy and dynamic and static light-scattering studies of ethidium bromide binding to calf thymus DNA: implications for outside-binding and intercalation The Journal of Physical Chemistry. ,vol. 96, pp. 6045- 6055 ,(1992) , 10.1021/J100193A073