The role of CD4+ cells in sustaining lymphocyte proliferation during lymphocytic choriomeningitis virus infection

摘要: The murine immune response to lymphocytic choriomeningitis virus (LCMV) infection involves the activation of CD8+, class I MHC-restricted and virus-specific CTL. At times coinciding with CTL activation, high levels IL-2 gene expression production occur, IL-2R is expressed, T cell blastogenesis proliferation are induced. We have previously found that, although both CD4+ CD8+ subsets transcribe IL-2, subset appears be major producer whereas proliferating population when separated after in vivo. studies presented here were undertaken examine contribution made by lymphocyte Responses LCMV examined intact mice depleted or antibody treatments Protocols such that vivo anti-CD4 anti-CD8 respective greater than 90%. In situ hybridizations demonstrated was expressed non-B lymphocytes isolated from either cell-depleted on day 7 post-infection LCMV. When placed culture, however, cells produced significantly higher detectable did post-infection. apparently cells, as for p55 chain IL-2R, responsiveness, observed sets mice. Lymphocyte shown sustained three criteria: 1) infected underwent more DNA synthesis those uninfected cells; 2) leukocyte yields expanded during mice; 3) numbers increased majority having characteristics blast recovered populations These findings suggest requirement sustain limited, types can function independently many aspects their responses viral infections.