Coculture of human oviductal cells maintains mitochondrial function and decreases caspase activity of cleavage-stage mouse embryos

作者: Jia-Sen Xu , Samuel Ting-Hon Chan , Pak-Chung Ho , William Shu-Biu Yeung

DOI: 10.1016/S0015-0282(03)00570-3

关键词:

摘要: Abstract Objective To investigate the mitochondrial function and caspase activity in mouse embryos after human oviductal cell coculture. Design Experimental laboratory study. Setting University gynecology unit. Animal(s) MF-1 (female); BALB/c (male) mice. Intervention(s) Mouse were cocultured with cells. Main outcome measure(s) Mitochondrial transmembrane potential (Δψ m ) activity. Result(s) Compared to coculture Chatot-Ziomek-Bavister (CZB) medium supplemented 0.5 mg/mL of BSA (CZB), Δψm cultured CZB was significantly lower at two-cell (CZB, 2.04 ± 0.412; coculture, 4.34 0.563) morula 6.06 0.548; 7.12 0.568) stages. Cocultured vivo developed had comparable Δψm. Caspase not detected unfragmented cleavage-stage vivo. In vitro possessed The reduced morula. Conclusion(s) Human cells maintained mitochondria terms decreased improve development embryo.

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