The population structure of clinical extra-intestinal Escherichia coli in a teaching hospital from Nigeria.
作者： Jeremiah Seni , Giselle Peirano , Kenneth Okwong Okon , Yusuf Bara Jibrin , Alkali Mohammed
DOI: 10.1016/J.DIAGMICROBIO.2018.04.001
关键词:
摘要: Limited information is available regarding the population structure of extra-intestinal pathogenic Escherichia coli (ExPEC) in Africa. Antimicrobial resistance profiles, sequence types (STs) and fimH were determined on 60 clinical ExPEC from Nigeria using a 7-single nucleotide polymorphism quantitative PCR sequencing certain genes. Different ST131 clades identified with multiplex PCR. The isolates mostly obtained urines (58.3%). Not-susceptibility rates as follows: trimethoprim-sulfamethoxazole (98%), cefotaxime (68%), gentamicin (55%), ciprofloxacin (62%) piperacillin-tazobactam (2%). Dominant STs associated CTX-M-15 included ST131-fimH30 (23%), ST457-fimH145 (20%), ST405-fimH27 (13%) ST95-fimH41 (10%). We found 7-SNP qPCR to be simple cost-effective that can utilized tract different clones global scale. This study provided insight into showing high prevalence rarely reported ST457 presence multidrug resistant ST95.
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