Distinct subsets of somatostatin receptors on cultured human lymphocytes.

摘要: Abstract Somatostatin (SOM) is a neuroendocrine tetradecapeptide that suppresses specific functions of differentiated T-cells and antibody-producing cells. The Jurkat line human leukemic U266 IgE-producing myeloma cells bound [I-Tyr11]SOM specifically. maximal level binding was attained by 1-2 h at 22 degrees C for both types reversed 70-85% within 2-3 after the addition excess nonradioactive SOM. Computer-assisted Scatchard analysis competition curves revealed two classes sites An average 144 1295 high affinity receptors per had Kd value 3 pM 5 pM, respectively, whereas large number low values 66 nM 100 nM. analogs somatostatin 28, [I-Tyr11]SOM, [D-Trp8, D-Cys14]SOM cell lines, relative to SOM, suggested degree specificity similar on