Human glutathione S-transferase theta (GSTT1): cDNA cloning and the characterization of a genetic polymorphism.

摘要: In humans, glutathione-dependent conjugation of halomethanes is polymorphic, with 60% the population classed as conjugators and 40% non-conjugators. We report characterization genetic polymorphism causing phenotypic difference. have isolated a cDNA that encodes human class Theta GST (GSTT1) which shares 82% sequence identity rat GST5-5. From PCR Southern blot analyses, it shown GSTT1 gene absent from 38% population. The presence or absence coincident conjugator (GSST1+) non-conjugator (GSTT1-) phenotypes respectively. GSTT1+ phenotype can catalyse glutathione dichloromethane, metabolic pathway has been to be mutagenic in Salmonella typhimurium mutagenicity tester strains believed responsible for carcinogenicity dichloromethane mouse. enzyme found erythrocyte this may act detoxification sink. Characterization will thus enable more accurate assessment health risk synthetic other industrial chemicals.