Long-term population studies uncover the genome structure and genetic basis of xenobiotic and host plant adaptation in the herbivore Tetranychus urticae

摘要: Pesticide resistance arises rapidly in arthropod herbivores, as can host plant adaptation, and both are significant problems agriculture. These traits have been challenging to study often polygenic many arthropods genetically intractable. Here, we examined the genetic architecture of pesticide adaptation two-spotted spider mite, Tetranychus urticae, a global agricultural pest. We show that short generation time high fecundity T. urticae be readily exploited experimental evolution designs for high-resolution mapping quantitative traits. As revealed by selection with spirodiclofen, an acetyl-CoA decarboxylase inhibitor, populations from cross between spirodiclofen resistant susceptible strain, which also differed performance on tomato, found limited number loci could explain this compound. were resolved narrow genomic intervals, suggesting specific candidate genes, including itself, clustered copy variable cytochrome P450 NADPH reductase, encodes redox partner P450s. For regions response distinct those responding synthetic compound consistent more architecture. In accomplishing work, continuous nature allele frequency changes across resolve existing fragmented draft genome pseudochromosomes. This improved assembly was indispensable our analyses, it will future research model herbivore is exceptionally amenable studies.